A Novel SNPs of KISS1 Gene Strongly Associated with Litter Size in Indonesian Goat Breeds

Kisspeptin is a protein encoded by the KISS1 gene which behaves as key role stimulating gonadotropin releasing hormone (GnRH) neuron activity directly in reproductive axis. The objective of present study was to determine genetic diversity within intron 1 and verify their association with fecundity traits can be devoted marker assisted selection (MAS) for breeding selection. This established on three Indonesian native goat populations (Kacang, Kejobong, Senduro). PCR products were then sequenced both directions. DNA sequencing alignment resulted fifteen variants (one indel fourteen SNPs), SNP1, SNP2, SNP3, SNP9 being novel single nucleotide polymorphisms (SNPs) 1. Genotype, haplotype, parity significantly associated litter size. SNP8, SNP9, SNP10 correlated overall means size (LS) LS at first third parities (p<0.05). Additionally, remarkably strong linkage disequilibrium SNP8 (D’=1.00; r≥0.58; χ2≥13.38), allele A had putative binding sites NFIC plays an important activating expression gene. H2 haplotype (CATAGCGCAACGCT) found have highest (p<0.0001). CC genotype AA GA SNP10, excellent genotypes that superior Therefore, this result led presume these SNPs acknowledged prominent markers goat-